Fig. 3: Only RB is indispensable for AURKBi-induced senescence. | Cell Death & Disease

Fig. 3: Only RB is indispensable for AURKBi-induced senescence.

From: Aurora B inhibitors promote RB hypophosphorylation and senescence independent of p53-dependent CDK2/4 inhibition

Fig. 3

A HCT116 cells with indicated genotypes were treated with 0.2 μM AZD2811 for two or six days. Cells were labelled with EdU for 2 h then fixed. Cells were then stained for EdU and Ki67 and >1000 cells per well were quantitated by high content imaging. The data represent the mean and SD of four replicates from at least two independent experiments. B Cells treated as in (A) were fixed and stained for senescence associated β-galactosidase activity (SA-β-Gal). Quantitation of the SA-β-Gal positive cells in the indicated HCT116 genotypes. The data are the mean and SD from quantitating >four fields per cell line, each with >100 cells/field. Comparisons were to the equivalent treatment point in the WT control using two-way ANOVA with Tukey’s multiple comparison test. *** p < 0.001, ****p < 0.0001. C NFF cells were treated as in (A) then either stained for SA-β-Gal or labelled with EdU and Ki67 then >2000 cells analysed by high content imaging.

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