Fig. 4: Loss of p53 only delays downregulation of critical cell cycle proteins. | Cell Death & Disease

Fig. 4: Loss of p53 only delays downregulation of critical cell cycle proteins.

From: Aurora B inhibitors promote RB hypophosphorylation and senescence independent of p53-dependent CDK2/4 inhibition

Fig. 4

A RB and p53 wild type HCT116 and HT1080 cells were treated for the indicated number of days with 0.2 μM AZD2811, then harvested and lysates immunoblotted for the indicated proteins. DREAM repressed cell cycle regulators (defined in [23]) are highlighted in red. This is representative of two independent experiments. B The levels of the DREAM repressed cell cycle regulators in (A) were quantified, expressed as percentage of control and combined. C The indicated HCT116 genotypes were treated for the indicated times with 0.2 μM AZD2811, harvested and lysates immunoblotted for the indicated proteins. The data are representative of replicate immunoblots. D The combined changes in cell cycle regulators from (C). E HCT116 wild type, RB deleted and RB+p53 deleted, MCF7 wild type and RB deleted lines were treated for the indicated times with 0.2 μM AZD2811 and immunoblotted for the indicated proteins. α-Tubulin was used as a loading control.

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