Fig. 5: AURKBi downregulates CDK4 and CDK2 activity. | Cell Death & Disease

Fig. 5: AURKBi downregulates CDK4 and CDK2 activity.

From: Aurora B inhibitors promote RB hypophosphorylation and senescence independent of p53-dependent CDK2/4 inhibition

Fig. 5

A HCT116 and HT1080 cells were treated for the indicated times with 0.2 μM AZD2811, then harvested and lysates immunoblotted for the indicated markers of CDK2 and CDK4 activity. The high mobility hypophosphorylated form of RB is indicated by the arrowhead in all panels. This is representative of replicate experiments. B The levels of phospho-RB and phospho-NPM were superimposed on the changes in the repressed cell cycle regulators (Fig. 4B). C The indicated HCT116 genotypes were treated for two or six days with 0.2 μM AZD2811 as in Fig. 4C and immunoblotted for RB and phospho-RB. D HCT116 wild type, RB deleted and RB and p53 deleted, MCF7 wild type and RB deleted lines were treated for the indicated times with AZD2811 and immunoblotted for the indicated proteins. The high mobility hypophosphorylated form of RB is indicated by the arrowhead. E HCT116 wild type and p53−/−, and HT1080 cells were treated without (Con) or with 0.2 μM AZD2811 for two days then harvested for immunoblotting for RB.

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