Fig. 5: MALAT1/SFPQ collaboratively promotes the SLC7A11 intron retention and ferroptosis sensitivity.
From: LncRNA MALAT1 promotes Erastin-induced ferroptosis in the HBV-infected diffuse large B-cell lymphoma

A The volcano plot showed that 987 genes were differentially expressed between high and low expression of MALAT1 in DLBCL tissue samples. B GSEA enrichment analysis of the HBV-regulated genes in DLBCL tissue samples. C Pearson correlation analysis between MALAT1 and SLC7A11 expression in the DLBCL patient cohorts (GSE10846). D The expression of SLC7A11 in DLBCL tissues and normal tissues. E Kaplan-Meier method for analyzing and comparing survival of individuals with high and low expression of SLC7A11. F ROC curve analysis for the predictive value of the combination of MALAT1, SFPQ, and SLC7A11 in the prognosis of DLBCL patients. G Schematic diagram of the primer design on SLC7A11 pre-mRNA and the intron 4 of SLC7A11 contains two TTGGTCT motifs. H The expression level of SLC7A11 transcript containing intron 4 after MALAT1 knockdown. I The expression level of SLC7A11 transcript containing intron 4 in the Ctrl, HBX, and HBX+MALAT1 cells. “*” represented the significance of HBX versus Ctrl. “#” represented the significance of HBX+MALAT1 versus HBX. “$” represented the significance of HBX+MALAT1 MUT versus HBX+MALAT1. J The SLC7A11 protein level in the Ctrl, HBX, HBX+MALAT1, and HBX+MALAT1 MUT cells. K Lipid peroxidation analysis for the effect of HBX, MALAT1, and MALAT1 MUT in SUDHL-4 cells after Erastin treatment. “*” represented the significance of HBX+Erastin versus Ctrl+Erastin. “#” represented the significance of HBX+MALAT1+Erastin versus HBX+Erastin. The results were determined in triplicate, and the error bars represented the mean ± SD. ns P > 0.05, ## P < 0.01, and ***/### P < 0.001.