Fig. 4: MIST1 knockout increased apoptosis in hepatocytes. | Cell Death & Disease

Fig. 4: MIST1 knockout increased apoptosis in hepatocytes.

From: MIST1 regulates endoplasmic reticulum stress-induced hepatic apoptosis as a candidate marker of fatty liver disease progression

Fig. 4

A Multi-dimensional scaling plot of RNA-sequencing datasets from non-treated Mist1KO mice (orange) and wild-type (WT) littermates (blue) and CCl4-treated Mist1KO mice (red) and WT littermates (green). B Gene set enrichment analysis comparing CCl4-treated Mist1KO and other groups at 8 weeks, (NES normalized expression score, p nominal p-value, FDR false discovery rate q-value). C Heatmap of gene set related to Hallmark of apoptosis. D Immunocytochemistry of cleaved caspase-3 in negative control (−) and MIST1 siRNA (+)-treated HepG2 cells with (+) or without (−) tunicamycin supplementation. E Percentage of cleaved caspase 3-positive cells over time in HepG2 cells; n = 4. All data are presented as mean ± SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA with Dunnett’s multiple comparison). F Western blot for cleaved caspase-3 in HepG2 cells cultured with (+) or without (−) tunicamycin, MIST1 siRNA (+), and/or control siRNA (−). All data are presented as mean ± SEM.

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