Fig. 3: AMT induces autophagy in glioma cells.
From: Amantadine against glioma via ROS-mediated apoptosis and autophagy arrest
A Immunofluorescent analysis of endogenous LC3 puncta in cells treated with or without AMT for 24 h. Scale bar: 10 μm. B, C Immunoblotting analysis of P62 expression and LC3 turnover, ATG7 and ATG5 in U87 and U251 cells treated with AMT for 24 h. D Immunoblotting analysis of AKT, p-AKT, mTOR, and p-mTOR, cells were treated with AMT for 24 h. E U87 and U251 cells were treated with AMT (40 μM) alone or in combination with 5 μM CQ for 24 h, and LC3 spots were detected by immunofluorescence assay. Scale bar 50 μm. F Cell growth of U87 and U251 cells treated with AMT (40 μM) alone or in combination with 5 μM CQ for 24 h (n = 3). G LDH release from U87 and U251 cells treated as in (E) for 24 h (n = 3). H Colony formation of U87 and U251 cells treated as in (E) for 24 h. Error bars indicate mean ± SD (**P < 0.01, ***P < 0.001).
