Fig. 3: GPR37 transcription is regulated by ZNF750 in ESCC. | Cell Death & Disease

Fig. 3: GPR37 transcription is regulated by ZNF750 in ESCC.

From: GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma

Fig. 3

A Correlation between ZNF750 expression and GPR37 expression in ESCA. B Western blot analysis of ZNF750 levels in KYSE410 and KYSE410R cells. C qRT‒PCR was used to detect the expression of ZNF750 in six ESCC cell lines (n = 3). D ZNF750 interacts with GPR37 promoter region. E Location of the binding site between ZNF750 and GPR37 DNA. F ChIP experiments showed that ZNF750 was enriched in GPR37. G Luciferase reporter gene detection suggested that GPR37 interacts with ZNF750. Western blotting (H) was used to detect the protein expression level of ZNF750, and qRT‒PCR (I) was used to detect the mRNA expression level of ZNF750 (n = 3). J qRT‒PCR was used to detect the expression level of the GPR37 mRNA (n = 3). K Western blotting was used to detect the protein expression level of GPR37 in cells after ZNF750 overexpression. ns not significant, *p < 0.05.

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