Fig. 6: GPR37 affects ESCC function and radiation tolerance by affecting the AKT/mTOR signaling pathway. | Cell Death & Disease

Fig. 6: GPR37 affects ESCC function and radiation tolerance by affecting the AKT/mTOR signaling pathway.

From: GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma

Fig. 6

A The GSEA results showed that GPR37 was negatively correlated with the mTOR signaling pathway. Western blotting was used to measure the levels of AKT/mTOR-related proteins in KYSE150 (B) and KYSE450 (C) cells. D Western blotting was used to detect changes in the KYSE150 cell protein after drug treatment. E A CCK8 assay was used to evaluate the proliferation of KYSE150 cells after drug treatment (n = 3). F Cell migration was measured after drug treatment of KYSE150 cells using a wound healing assay (n = 3). The wound width at 0 h was normalized. Scale bar: 100 μm. G Cell migration was measured after drug treatment of KYSE150 cells using a transwell assay without Matrigel (n = 3). Scale bar: 100 μm. H Transwell assays with Matrigel were used to measure the invasion of KYSE150 cells after drug treatment (n = 3). Scale bar: 100 μm. Flow cytometry was used to evaluate the apoptosis of SC79 (I)- and MHY1485 (J)-treated KYSE150 cells after 8 Gy irradiation (n = 3). ns not significant. *p < 0.05, ***p < 0.001, ****p < 0.0001.

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