Fig. 7: GPR37 promotes ubiquitinated degradation of ATP1A1.

A Silver staining showing a sample of GPR37 co-IP. B Western blot analysis of endogenous Co-IP samples. C Western blot analysis of exogenous Co-IP samples. D Immunofluorescence detection of the location of GPR37 and ATP1A1 in KYSE150 cells. Scale bar: 10 μm. E Western blotting and qRT-PCR were used to detect the expression level of ATP1A1 in KYSE150 cells (n = 3). F Western blotting was used to detect the changes in the levels of GPR37 and ATP1A1 protein in 293 T cells after transfection with GPR37 overexpression plasmid, and the relative protein expression levels were statistically analyzed compared with the control group (0 h). G Western blotting was used to detect the ATP1A1 protein level after MG132 treatment. H The ubiquitination level of ATP1A1 in cells with different GPR37 expression levels was detected. I qRT-PCR was used to detect the mRNA expression level of Parkin (n = 3). J Western blotting was used to detect the ubiquitination level of ATP1A1 in cells after co-transfection of different plasmids. K Western blotting was used to detect the protein levels in KYSE150 cells overexpressing ATP1A1. **p < 0.01.