Fig. 1: LINC01016 is upregulated in GC tissues with LNM and is associated with GC progression.

A LINC01016 is highly expressed in GC tissues with LNM (n = 51) than in those without LNM (n = 29). B ROC curve is used to identify that LINC01016 could be used to distinguish the patients with LNM. C LINC01016 is notably upregulated in GC cell lines, especially in HGC27 and BGC823 cell lines. D RNA FISH assays reveals that LINC01016 is primarily located in the nucleus. U6 and 18S are utilized as controls for these localization analyses. E LINC01016, U6, and GAPDH levels are assessed in nuclear and cytoplasmic GC cell fractions by qRT-PCR. F–G GC patients in the high-LINC0106 group (n = 55) have shorter overall survival (OS) and relapse-free survival (RFS) than those in the low-LINC01016 group (n = 25). Higher than median LINC01016 expression is defined as high expression. On the contrary, the definition is low expression. Scale bar: 100μm. *P < 0.05, **P < 0.01,***P < 0.001.