Fig. 4: EIF4A3 is a nuclear binding protein of LINC01016. | Cell Death & Disease

Fig. 4: EIF4A3 is a nuclear binding protein of LINC01016.

From: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay

Fig. 4

A A number of proteins in the range of 43–55 KDa that were co-precipitated by the LINC01016 sense transcript but not by the LINC01016 antisense transcript via pull-down assay and silver staining. B EIF4A3 is ultimately identified as a LINC01016-interacting protein by western blotting. C The binding of LINC01016 and EIF4A3 is confirmed via RIP assay, with qRT-PCR products being verified via agarose electrophoresis. D Truncated biotin-linked LINC01016 are used to pull down cellular protein and reveal that 1217–1857 nucleotide (nt) region could bind to EIF4A3. *P < 0.05, **P < 0.01,***P < 0.001.

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