Fig. 3: LPS-treated WT and Ezr-KO^m lung MΦs have an altered expression profile consistent with a lack of differentiation toward a tissue-resident macrophage phenotype.

A Multidimensional scaling (MDS) plot depicting the distinct clusters of moMs, tnsIMs and matIMs in WT and Ezr-KO^m mice based on RNA expression profiles. B Comparative expression of cellular markers and their transition gene expression profiles in moMs, tnsIMs and matIMs between WT and Ezr-KO^m mice treated with LPS. C Hierarchical clustered heatmap depicting RNA expression profiles of differentially expressed genes (DEGs) comparing moMs, tnsIMs and matIMs in WT and Ezr-KO^m mice treated with LPS. Each tile represents the triplicate average of normalized fragments per kilobase of transcript per million mapped (FPKM) reads and colored according to gene-specific z-scores. Data were generated from three mice per genotype. Data are represented as median from three biological replicates. Statistical analysis was performed using a t-test (non-parametric) between the subpopulations. *p < 0.05, **p < 0.01, ***p < 0.001 and ns non significant. See also Supplementary Fig. S6.