Fig. 6: Hpa2-KO macrophages exert pro-tumorigenic properties.
A Matrigel plug. Macrophages were isolated from thioglycolate-treated WT and Hpa2-KO mice and were suspended in Matrigel (3 × 106/ml). Matrigel containing WT (Mat+WT) or Hpa2-KO (Mat+KO) macrophages was injected subcutaneously (0.5 ml/mouse) in C57Bl/6 mice; Matrigel plugs were collected 10 days thereafter, fixed in formalin, and embedded in paraffin. 5-micron sections were subjected to immunostaining applying anti-F4/80 (upper panels), anti-SMA (second panels), and anti-CD31 (third and fourth panels) antibodies. Original magnifications: ×10 (scale bars represent 500 microns); ×100 (lower right panel; scale bars represent 50 microns). B Immunostaining. 5-micron sections of the indicted tumor xenograft were subjected to immunostaining by applying antibody directed against F4/80 (a marker for macrophages) shown at low (×10, left panels; scale bars represent 500 microns) and high (×100, right panels; scale bars represent 50 microns) magnifications. Note increased recruitment of macrophages by tumors produced by cells overexpressing Hpa2 and Hpa2 mutants.
