Fig. 1: TIP60 and ΔNp63α are overexpressed in cisplatin-resistant cells.
From: TIP60 enhances cisplatin resistance via regulating ΔNp63α acetylation in SCC
A A431 Parental and A431 Pt and B JHU029 and JHU006 cells were subjected to a 2-h cisplatin pulse treatment at the indicated doses. At 48-h post treatment, cell viability was measured by MTS assay (left panel). The y-axis indicates cell viability relative to vehicle-treated cells. The x-axis indicates the μg/μl concentration of cisplatin used for pulse treatment. Error bars represent ±1 SD from the mean. *p < 0.05 compared to respective control at each dose of cisplatin. Bar plots (right panel) show the mean IC50 value calculated from three independent experiments. Error bars indicate the mean +1 SEM from three independent experiments. *p < 0.05 compared to the IC50 value of A431 Parental or JHU029 sensitive controls. Immunoblot analysis performed on C A431 Parental and A431 Pt D JHU029 and JHU006 cells using antibodies specific for p63 and TIP60. Fold change in ΔNp63α protein relative to respective control is listed above each band. β-actin was included as a loading control. Representative blots are shown.
