Fig. 3: Marcks overexpression does not alter the mTOR, GSK3β, and STAT3 pathways.

A Representative images of retinal sections showing that Marcks overexpression did not activate mTOR (marked by p-S6) in RGCs 3 days after optic nerve crush. Retinal sections were stained with RBPMS (red), and p-S6 (green). Scale bar: 20 μm. B Quantification of fluorescence intensity of p-S6 in RGCs shown in (A). ns no significance. RGCs were analyzed from at least 7 non-adjacent retinal sections for each animal, from 3 mice per group. C Representative images of retinal sections showing that Marcks overexpression did not inactive GSK3β (marked by p-GSK3β) levels in RGCs 3 days after optic nerve crush. Retinal sections were stained with RBPMS (red), and p-GSK3β (green). Scale bar: 20 μm. D Quantification of fluorescence intensity of p-GSK3β in RGCs shown in (C). ns no significance. RGCs were analyzed from at least 10 non-adjacent retinal sections for each animal, from 3 mice per group. E Representative images of retinal sections showing that Marcks overexpression did not increase STAT3 phosphorylation levels in RGCs 3 days after optic nerve crush. Retinal sections were stained with RBPMS (red), and p-STAT3 (green). Scale bar: 20 μm. F Quantification of fluorescence intensity of p-STAT3 in RGCs shown in (E). ns no significance. RGCs were analyzed from at least 9 non-adjacent retinal sections for each animal, from 3 mice per group.