Fig. 1: SHEP1 selectively expressed in macrophages co-cultured with H/R cardiomyocytes fluctuates over time.

A Representative dual-immunofluorescence staining of SHEP1(green), F4/80(red), and nuclei (4,6-diamidino-2-phenylindole, blue) in mice hearts 1 day after IR. Scale bar, 10 μm. B Flow chart of the co-culture system. SHEP1 mRNA levels (C) and protein levels (D) in RAW264.7 co-cultured with hypoxia-reoxygenated cardiomyocytes for different time periods (n = 3 sets of cells). Data are presented as mean ± SEM.