Fig. 3: SHEP1 regulates macrophages activities primarily through the MAPK signaling pathway.

A RNA-sequencing (RNA-seq) for total RNA extracted from RAW264.7 cells (SHEP1 KO and WT) co-cultured for 0 h (SH) or 6 h (H6) with H/R primary cardiomyocytes isolated from adult mice. VENN, volcano blots, KEGG enrichment analysis and Heatmap are displayed. B WAVE2 and p-ERK1/2 protein levels in SHEP1 knocked-out (KO)/over-expressed (OE) RAW264.7 and their respective negative controls (NC-KO and NC-OE) co-cultured with H/R primary cardiomyocytes from adult mice for 9 or 24 h (n = 3 sets of cells). All data are presented as mean ± SEM.