Fig. 5: The expression of SHEP1 shows sequential characteristic under the condition of MIRI in vivo. | Cell Death & Disease

Fig. 5: The expression of SHEP1 shows sequential characteristic under the condition of MIRI in vivo.

From: SHEP1 alleviates cardiac ischemia reperfusion injury via targeting G3BP1 to regulate macrophage infiltration and inflammation

Fig. 5

A Representative immunohistochemical analyses of SHEP1 at different time points after IR. Scale bar, 500 μm. SHEP1 expression were quantified and compared (n = 4). B Representative immunohistochemical analyses of SHEP1 in remote area, border area, and infarct area on day 1 after IR. Scale bar, 500 μm. SHEP1 expression were quantified and compared (n = 4). C S SHEP1 mRNA levels were analyzed by RT-qPCR at different time points after MIRI (n = 4). D SHEP1 protein levels in myocardium were examined at different time points after MIRI by western blot with SHEP1 antibody, β-actin was used as loading control (n = 4). All data are presented as mean ± SEM.

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