Fig. 6: Macrophage-specific deficiency of SHEP1 deteriorated cardiac function and cardiac injury under MIRI in vivo. | Cell Death & Disease

Fig. 6: Macrophage-specific deficiency of SHEP1 deteriorated cardiac function and cardiac injury under MIRI in vivo.

From: SHEP1 alleviates cardiac ischemia reperfusion injury via targeting G3BP1 to regulate macrophage infiltration and inflammation

Fig. 6

A Representative cardiac echocardiography images at 3 and 21 days after MIRI with quantification of percentage ejection fraction (% EF) and percentage fractional shortening (% FS) (n = 12) (B) Representative heart sections from LysMCre+Shep1fl/fl or Shep1fl/fl mice stained with Evans-blue and 2,3,5-triphenyltetrazolium chloride (TTC) at 1 day after MIRI to delineate the area at risk (AAR) and the infarcted region. The ratios of AAR/LV and infarct area/AAR were compared (n = 6). C Representative photomicrographs of terminal deoxynucleotidyl transferase–mediated deoxyuridinetriphate nick-end labeling (TUNEL) and nuclear (DAPI) staining of myocardium obtained 1day after MIRI. TUNEL-positive cell number per ROI were compared (n = 6). D Representative Western blot analysis showing the protein levels of Bax and Bcl-2 in LysMCre+Shep1fl/fl and Shep1fl/fl mice hearts at 1 day after MIRI or sham operation (n = 6). E Representative Masson trichrome staining of cardiac tissue obtained from LysMCre+Shep1fl/fl and Shep1fl/fl mice at day 21 after MIRI. Quantitative analysis of fibrotic areas (n = 6). All data are presented as mean ± SEM.

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