Fig. 7: Macrophage-specific deficiency of SHEP1 exacerbates cardiac injury through amplifying inflammatory process under MIRI in vivo. | Cell Death & Disease

Fig. 7: Macrophage-specific deficiency of SHEP1 exacerbates cardiac injury through amplifying inflammatory process under MIRI in vivo.

From: SHEP1 alleviates cardiac ischemia reperfusion injury via targeting G3BP1 to regulate macrophage infiltration and inflammation

Fig. 7

A Representative dual-immunofluorescence staining of SHEP1 (green), F4/80 (red), and nuclei (4,6-diamidino-2-phenylindole, blue) in mice hearts (flox and CKO) 1 and 3 days after IR, F4/80+ (red) cell numbers were counted and compared (n = 4; scale bar, 500 μm). B, C Flow cytometry analysis for cardiac macrophages within LV myocardium in LysM Cre+ Shep1fl/fl and Shep1fl/fl mice 1 or 3 days after MIRI. n = 5 mice/group pooled from 5 independent experiments. D RT-qPCR analysis of the mRNA levels of inflammatory mediators in cells sorted by F4/80 magnetic beads from total cardiac cells 1 or 3 days after MIRI from LysM Cre+ Shep1fl/fl mice and their Shep1fl/fl littermates. All data are presented as mean ± SEM.

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