Fig. 1: Binding sites of ASPP family members and the DBDs of p53 family members.
From: Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro
A Superimposed structures of the p53 DBD (grey) bound to DNA (light blue), ASPP2 CTD (green) and iASPP CTD (purple). The SH3 domain and the ankyrin repeats of the CTDs are shown in light and dark colour, respectively. The residue R248 of p53 (orange) directly contacts DNA and the SH3 domain of ASPP2. Zinc is displayed as a grey sphere (PDB: 2AC0, 1YCS and 6RZ3). B Sequence alignment of the p53 family DBDs and the proximate linker region. The secondary structure elements of the DBDs are shown and labelled accordingly. Residues involved in binding ASPP2 CTD and iASPP CTD are shaded in green and purple, respectively. The arginine (p53: R248, p63: R279, p73: R268) involved in the interaction with DNA and ASPP2 is highlighted in orange. The linker region (black box) of p53 is known to be bound by iASPP CTD.
