Fig. 4: The linker region modulates the interaction with members of the ASPP family.
From: Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro
A Pull-down assay of ΔNp63α WT and indicated variants with GST-tagged ASPP2 (grey) and iASPP CTDs (white) as bait. N-terminally Myc-tagged proteins were in-vitro translated using RRL. Input and pull-down (PD) samples were analysed by WB using an α-Myc antibody. For the relative pull-down efficiency, the PD signals were normalized to the IP signals and p63 WT was set to 1 for ASPP2 and iASPP individually. B Pull-down assay of ΔNp73α WT and indicated variants with GST-tagged iASPP CTD as bait. N-terminally Myc-tagged proteins were in-vitro translated using RRL. Input and pull-down (PD) samples were analysed by WB using an α-Myc antibody. For the relative pull-down efficiency, the PD signals were normalized to the IP signals and p73 WT was set to 1. A, B The bar diagrams show the mean relative pull-down efficiency and error bars the corresponding SD (n = 3). Statistical significance was assessed by ordinary one-way ANOVA (n.s.: P > 0.05, *: P ≤ 0.05, **: P ≤ 0.01, ***: P ≤ 0.001, ****: P ≤ 0.0001).
