Fig. 4: Rnd3 overexpression reversed Dox-induced cardiomyocyte apoptosis, necroptosis, and pyroptosis (PANoptosis). | Cell Death & Disease

Fig. 4: Rnd3 overexpression reversed Dox-induced cardiomyocyte apoptosis, necroptosis, and pyroptosis (PANoptosis).

From: Rnd3 protects against doxorubicin-induced cardiotoxicity through inhibition of PANoptosis in a Rock1/Drp1/mitochondrial fission-dependent manner

Fig. 4

A Gene set enrichment analysis, based on Wiki pathways, GO, KEGG and REACTOME, showing apoptosis, pyroptosis, and necroptosis signaling pathways (PANoptosis). B Representative immunofluorescence images showing YP1-positive cells (green) which may undergo apoptosis or necroptosis and PI-positive cells (red) which may undergo necroptosis, or pyroptosis, scale bar = 50 μm. C Quantitative analysis of YP1 and PI-positive cells (n = 5). D Representative Western blot images of NRLP3, ZBP1, AIM2, Cleaved-casp8, Casp8, Cleaved-casp3, Casp3, N-GSDMD, FL-GSDMD, Cleaved-casp1, Casp1, p-RIPK1, t-RIPK1, p-RIPK3, t-RIPK3, p-MLKL, and t-MLKL protein levels. E–N Quantitative analysis of NRLP3, ZBP1, AIM2, Cleaved-casp8/Casp8, Cleaved-casp3/Casp3, N-GSDMD/FL-GSDMD, Cleaved-casp1/Casp1, p-RIPK1/t-RIPK1, p-RIPK3/t-RIPK3, and p-MLKL/t-MLKL protein expression (n = 5). Data were presented as mean ± SD. One-way ANOVA was used for statistical analysis in (C), (E), and (F)–(N).

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