Fig. 5: Rock1 played a pivotal role in the Rnd3-induced mitigation of DIC. | Cell Death & Disease

Fig. 5: Rock1 played a pivotal role in the Rnd3-induced mitigation of DIC.

From: Rnd3 protects against doxorubicin-induced cardiotoxicity through inhibition of PANoptosis in a Rock1/Drp1/mitochondrial fission-dependent manner

Fig. 5

A–C IntAct, STRING and GeneMANIA databases indicated Rock1 as a potential interacting protein with Rnd3. D Representative Western blot images of RhoA/Rock pathway related protein in cardiomyocytes from Ad-Control or Ad-Rnd3 group. E Quantitative analysis of RhoA/Rock pathway related protein expression (n = 5). F Representative immunofluorescence images of Rnd3 and Rock1 in cardiomyocytes. And quantitative analysis of the colocalization between Rnd3 and Rock1. G Cell lysates of primary cardiomyocytes were immunoprecipitated with IgG, Rnd3 or Rock1 antibodies, and Western blot were performed using Rnd3 and Rock1 antibodies. H Structure-based protein interaction interface analysis between Rnd3 and Rock1 using the HDOCK server. I The docking sites of Rnd3 and Rock1. J–L Interaction among Rnd3, Rock1 and Drp1 in cardiac tissues was examined by IP-Western blot assay. M Interaction among Rnd3, Rock1 in cytoplasm or mitochondrial examined by IP-Western blot assay. Data were presented as mean ± SD. One-way ANOVA was used for statistical analysis in (E).

Back to article page