Fig. 6: Rnd3 bond to Rock1 to mediate Dox-induced mitochondrial fission. | Cell Death & Disease

Fig. 6: Rnd3 bond to Rock1 to mediate Dox-induced mitochondrial fission.

From: Rnd3 protects against doxorubicin-induced cardiotoxicity through inhibition of PANoptosis in a Rock1/Drp1/mitochondrial fission-dependent manner

Fig. 6

A Cardiomyocytes were infected with siRNA-control, siRNA-Rock1, Ad-Control, or Ad-Rnd3, and then treated with Dox (2 μM) for the indicated time periods. Representative Western blot images of Drp1-p616, and total Drp1 protein levels for each group. B Quantitative analysis of Drp1-p616/Drp1 protein expression (n = 5). C Representative mitochondrial images of Mito tracker staining obtained by confocal microscope, scale bar = 10 μm. D Quantitative analysis of mitochondrial morphology. E Representative immunofluorescence images of JC-1 staining, scale bar = 10 μm. F Quantitative analysis of the ratio of aggregated and monomeric JC-1 (n = 5). G Representative Mito-SOX fluorescence images indicate the level of mitochondrial oxidative stress, scale bar = 10 μm. H Quantitative analysis of mtROS production (n = 5). Data were presented as mean ± SD. One-way ANOVA was used for statistical analysis in (B), (D), (F), and (H).

Back to article page