Fig. 6: Death receptor-induced, but not IL-1β-induced gene induction is severely affected in TRAF2-RIPK1 double deficient cells. | Cell Death & Disease

Fig. 6: Death receptor-induced, but not IL-1β-induced gene induction is severely affected in TRAF2-RIPK1 double deficient cells.

From: TRAF2 and RIPK1 redundantly mediate classical NFκB signaling by TNFR1 and CD95-type death receptors

Fig. 6

A The indicated HCT116-PIK3CAmut cell line variants were challenged with TNF (100 ng/ml, 18 h) and analyzed by NGS. B Cells were stimulated overnight (16–18 h) with 100 ng/ml TNF, 200 ng/ml Fc-CD95L or 20 ng/ml IL-1β to prepare total RNA. The latter was reverse transcribed to cDNA and analyzed by qPCR with respect to the expression of the indicated mRNAs (normalized to RPLP0). qPCR data shown are derived from 4 independent experiments and were statistically evaluated by one-way ANOVA (Bonferroni post-hoc test). ***p < 0.001; **p < 0.01; *p < 0.05.

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