Fig. 3: Silencing of KLF5 potentiates ferroptosis by upregulating HMOX1. | Cell Death & Disease

Fig. 3: Silencing of KLF5 potentiates ferroptosis by upregulating HMOX1.

From: Inhibition of KLF5 promotes ferroptosis via the ZEB1/HMOX1 axis to enhance sensitivity to oxaliplatin in cancer cells

Fig. 3

A Venn diagram showing that the ferroptosis-related genes downstream of KLF5 intersected from the ferroptosis dataset and KLF5-mediated TCGA-PAAD cohort and transcriptome sequencing. B mRNA level in human PDAC cells treated by control plasmid or shKLF5. C WB showed the change in HMOX1 caused by individually or simultaneously suppressed KLF5 and HMOX1 in CFPAC-1 and Capan-1 cells. D KLF5 and HMOX1 in CFPAC-1 and Capan-1 cells were individually or simultaneously suppressed and cell viability was evaluated using calcein-AM. E, F BODIPY 581/591 C11 was used to detect lipid peroxidation in PDAC cells treated by shKLF5 or shHMOX1 or both. G Lipid peroxidation of PDAC cells treated by shKLF5 or shHMOX1 or both, detected using confocal microscopy. H MDA was detected in PDAC cells treated by shKLF5 or shHMOX1 or both.

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