Fig. 1: Phenotypic and functional characterization of HLC derived from hESC. | Cell Death & Disease

Fig. 1: Phenotypic and functional characterization of HLC derived from hESC.

From: Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease

Fig. 1

a Representative bright field images of the differentiation phases that lead WA01 and WA09 hESC to differentiate into HLC. Scale bar, 40 µm. b hESC, HLC and LPO-treated HLC were subjected to qRT-PCR analysis. The relative quantity is shown using ∆Ct. Data represent means ± SEM. One-way ANOVA, Tukey-corrected. **p < 0.01; ***p < 0.001. Each dot represents a biological replicate, n = 3. c Total protein lysates from WA01 and WA09 hESC, derived HLC and LPO-treated HLC were subjected to western blot analysis with the antibodies indicated. The Histone H3 (H3) is used as a protein loading control normalizer.

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