Fig. 2: Lactate, pyruvate, and octanoic acid-induced steatosis in HLC and immortalized cells. | Cell Death & Disease

Fig. 2: Lactate, pyruvate, and octanoic acid-induced steatosis in HLC and immortalized cells.

From: Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease

Fig. 2

Untreated and LPO-exposed a WA01 and WA09 HLC, b AML12, and HepG2 cells were subjected to confocal analysis as indicated in the figure. Representative confocal images of BODIPY493/503 stained cells are shown (orange/yellow: LD; blue: DAPI, nuclei. Scale bar, 25 µm). Quantification of LD number/cell and their average size (arbitrary unit) were reported. The confocal analysis of LPO-treated AML12 and HepG2 cells has been used as reference in Fig. 5b. c Untreated and LPO-exposed WA01, WA09, AML12, and HepG2 cells were subjected to qRT-PCR analysis. The relative quantity normalized on untreated is shown using ∆∆Ct for AML12 and HepG2 cells, whereas the relative quantity is shown using ∆Ct for WA01 and WA09 cells. Data represent means ± SEM. (a—n° lipid droplets; b; c—AML12 and HepG2) Student’s t-test, (a—average size) Mann–Whitney, (c—HLC) Kruskal–Wallis, Dunnett-corrected, *p < 0.05, ***p < 0.001, ****p < 0.0001. Each dot represents a biological replicate, n ≥ 3.

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