Fig. 5: Estrogens reduced LD accumulation in LPO-treated hepatocytes.
a LPO-treated WA01 and WA09 HLC were cultured with or without 1 nM of E2 for 48 h and subjected to confocal analysis. Representative confocal images of BODIPY493/503 stained cells are shown (orange/yellow: LD; blue: DAPI, nuclei. Scale bar, 25 µm). Quantification of BODIPY493/503 spots/cell is reported. b LPO-treated AML12 and HepG2 were cultured with or without 50 nM of E2 for 48 h and subjected to confocal analysis. Representative confocal images of BODIPY493/503 stained cells are shown (orange/yellow: LD; blue: DAPI, nuclei. Scale bar, 25 µm). Quantification of LD number/cell and their average size (arbitrary unit) is reported. The confocal analysis of LPO-treated AML12 cells and HepG2 cells have been used as reference in Fig. 2b and has been processed in parallel with E2-treated cells. c LPO-exposed AML12 cells were treated with 50 nM of E2 for 48 h and 20 µM of ATGL inhibitor (ATGLi) for 24 h. Representative confocal images of BODIPY493/503 stained cells are shown (orange/yellow: LD; blue: DAPI, nuclei. Scale bar, 25 µm). Quantification of LD number/cell and their average size (arbitrary unit) is reported. Data represent means ± SEM. a–c Student’s t-test, **p < 0.01, ***p < 0.001, ****p < 0.0001. a–c n ≥ 3 in three technical replicates.
