Fig. 2: ATF4 and IRE1α are involved in ischemic tumor cell death. | Cell Death & Disease

Fig. 2: ATF4 and IRE1α are involved in ischemic tumor cell death.

From: Involvement of p38 MAPK and MAPKAPK2 in promoting cell death and the inflammatory response to ischemic stress associated with necrotic glioblastoma

Fig. 2

A Differentially expressed genes when comparing cells under the ischemic condition to those under the regular condition. B Ingenuity Pathway Analysis identified upregulated cellular stress pathways. C mRNA levels of each indicated gene in LN229 cells under regular (control) and ischemic conditions. D LN229 cells cultured under regular (control) or ischemic conditions were lysed and subjected to western blotting. E Representative TEM images from LN229 cells cultured under regular (control) or ischemic conditions. Arrows and arrowheads point to mitochondria and ER, respectively. F, G LN229 cells stably transduced by indicated shRNAs targeting ATF4 or a scrambled shRNA control were cultured in the ischemic condition for 18 h and subjected to crystal violet cell viability assay (F), or western blotting (G). H, I LN229 cells stably transduced by indicated shRNAs targeting IRE1α or a scrambled shRNA control were cultured in the ischemic condition for 18 h and subjected to crystal violet cell viability assay (H), or q-RT-PCR (I). J Phase contrast images of LN229 cells transduced by indicated shRNAs and cultured under ischemic conditions for 18 h. For C, Student’s t test. For F, H and I Ordinary one-way ANOVA test.

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