Fig. 4: The connection between p38-MK2 signaling and UPR regulators.

A LN229 cells stably transduced by indicated shRNAs targeting IRE1α or a scrambled shRNA control were cultured under the ischemic condition and subjected to western blotting. B LN229^vector, LN229^KRAS(G12D) or LN229^{PIK3CA(H1047R)} cells transfected by a pool of four siRNA against IRE1α or scrambled siRNA and cultured under regular or ischemic conditions were subjected to western blotting. C, D LN229 cells stably transduced by indicated sgRNAs targeting p38 (C) or MK2 (D), or an empty vector (EV) were cultured under regular (control) or ischemic conditions and subjected to western blotting. E A diagram illustrating the signaling pathways activated under the ischemic condition.