Fig. 1: Inflammatory stimulation decreased RNF128 expression in macrophages. | Cell Death & Disease

Fig. 1: Inflammatory stimulation decreased RNF128 expression in macrophages.

From: RNF128 deficiency in macrophages promotes colonic inflammation by suppressing the autophagic degradation of S100A8

Fig. 1

A Representative images of immunofluorescence co-staining for RNF128 (green) and the indicated cell-type markers (red) in human colitis tissues. DAPI (blue) was used to label the nuclei. Scale bars, 10 μm. B Representative images of immunofluorescence co-staining for Rnf128 (green) and the indicated cell-type markers (red) in colitis tissues from DSS-induced colitis mice. DAPI (blue) labels the nuclei. Scale bars, 10 μm. C Relative RNF128 mRNA levels (normalized to GAPDH RNA levels) in PBMCs, BMDMs and THP-1 cells stimulated with LPS for different periods of time. Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. D Western blot analysis of RNF128 protein levels in PBMCs, BMDMs and THP-1 cells after LPS stimulation for different periods of time. E Relative RNF128 mRNA levels (normalized to GAPDH RNA levels) in peripheral blood monocytes from healthy (n = 20) and patients with colitis (n = 20). Statistical data are presented as mean ± SD. **P < 0.01. F Relative Rnf128 mRNA levels (normalized to GAPDH RNA levels) in BMDMs from control (n = 5) and DSS-induced colitis mice (n = 5). Statistical data are presented as mean ± SD. **P < 0.01. G Western blot analysis of Rnf128 protein levels in BMDMs from control (n = 5) and DSS-induced colitis mice (n = 5).

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