Fig. 2: Rnf128 deficiency aggravated DSS-induced colon injury. | Cell Death & Disease

Fig. 2: Rnf128 deficiency aggravated DSS-induced colon injury.

From: RNF128 deficiency in macrophages promotes colonic inflammation by suppressing the autophagic degradation of S100A8

Fig. 2

A Experimental scheme of DSS-induced colitis model. The mice were treated with 2.5% DSS drinking water for 7 consecutive days. B Representative images of colons from Rnf128+/+ (n = 5) and Rnf128−/− (n = 5) mice after DSS treatment for 7 days. Scale bars, 1 cm. C The colon length in (B) was measured. Statistical data are presented as mean ± SD. ns nonsense, ***P < 0.001. D The body weight of the mice in (B) was measured daily. Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01. E Disease activity index scores of the mice in (B). Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01. F Survival analysis of Rnf128+/+ and Rnf128−/− mice treated with water or 3% DSS (n = 10). Statistical data are presented as mean ± SD. *P < 0.05. G Representative H&E-staining images of colon sections from the mice in (B). Scale bar, 500 μm. H Histopathology score in (G) was measured. Statistical data are presented as mean ± SD. ns, nonsense, **P < 0.01. I Representative periodic acid sthiff-alcian blue (PAS/AB) staining images of colon sections from mice in (B). Scale bars, 500 μm. J The PAS-positive areas in (I) were measured and analyzed. Statistical data are presented as mean ± SD. ns, nonsense; **P < 0.01. K Representative Ki67 staining images of colon sections from mice in (B). Scale bars, 500 μm. L The Ki67 positive areas in (K) were measured and analyzed. Statistical data are presented as mean ± SD. ns nonsense. *P < 0.05.

Back to article page