Fig. 3: RNF128 deficiency augments macrophage infiltration and inflammatory factors production. | Cell Death & Disease

Fig. 3: RNF128 deficiency augments macrophage infiltration and inflammatory factors production.

From: RNF128 deficiency in macrophages promotes colonic inflammation by suppressing the autophagic degradation of S100A8

Fig. 3

A Rnf128+/+ (n = 5) and Rnf128−/− (n = 5) mice were exposed to 2.5% DSS for 7 days. Representative histochemical images of Cd68 staining in distal colon sections. Scale bars, 500 µm. B The staining positive areas in (A) were measured and analyzed. Statistical data are presented as mean ± SD. *P < 0.05. C Rnf128+/+ (n = 5) and Rnf128−/− (n = 5) mice were exposed to 2.5% DSS for 7 days. Representative histochemical images of F4/80 staining in distal colon sections. Scale bar, 500 µm. D The F4/80 positive areas in (C) were measured and analyzed. Statistical data are presented as mean ± SD. **P < 0.01. E Rnf128+/+ (n = 5) and Rnf128−/− (n = 5) mice were exposed to 2.5% DSS for 7 days. Representative histochemical images of Ly6C high staining in distal colon sections. Scale bars, 500 µm. F The Ly6C positive areas in (E) were measured and analyzed. Statistical data are presented as mean ± SD. *P < 0.05. G Rnf128+/+ (n = 5) and Rnf128−/− (n = 5) mice were exposed to 2.5% DSS for 7 days. Representative histochemical images of Ly6G high staining in distal colon sections. Scale bars, 500 µm. H The Ly6G positive areas in (G) were measured and analyzed. Statistical data are presented as mean ± SD. ns nonsense. I The mRNA levels of Il18, Il6, Il1β, Il1α and Tnf-α (each normalized to GAPDH RNA level) in colon tissues from Rnf128+/+ (n = 5) and Rnf128–/– (n = 5) mice subjected to 2.5% DSS. Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. J BMDMs were extracted from Rnf128+/+ and Rnf128–/– mice and stimulated with LPS (200 ng/ml) for 8 h. The mRNA levels of Il18, Il6, Il1β, Il1α and Tnf-α (each normalized to GAPDH RNA level) in the BMDMs were detected via qRT‒PCR. The statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01. K ELISA analysis of IL-1β and TNF-α levels in colon tissue from Rnf128+/+ (n = 5) and Rnf128–/– (n = 5) mice subjected to 2.5% DSS. Statistical data are presented as mean ± SD. *P < 0.05. L ELISA analysis of IL-1β and TNF-α levels in the supernatant of BMDMs. Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01.

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