Fig. 5: piR-26441 inhibits OXPHOS in OC cells and activates ROS to initiate the apoptotic cascade. | Cell Death & Disease

Fig. 5: piR-26441 inhibits OXPHOS in OC cells and activates ROS to initiate the apoptotic cascade.

From: piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1

Fig. 5

a qRT-PCR results showed the RNA levels of TSFM decreased in CAOV3 and OVCAR3 cells after piR-26441 overexpression, which was contrary to that after knockout. b After treatment with actinomycin D (5 μg/ ml) for 0, 1, 2, and 3 h, RT-qPCR assay showed overexpression of piR-26441 shortened the mRNA half-life of TSFM in CAOV3 cells, which was contrary to that after knockout. c Western blot results showed TSFM and NDUFB8 protein levels decreased in CAOV3 and OVCAR3 cells after overexpression of piR-26441, which was contrary to that after knockout. d JC-1 staining showed that overexpression of piR-26441 decreased the mitochondrial membrane potential of CAOV3, while knockdown did the opposite. e The results of R01 fluorescent probe showed the oxygen consumption rate decreased in CAOV3 cells after piR-26441 overexpression, which was contrary to that after knockout. f Flow cytometry analysis and fluorescence microscopy results showed ROS levels increased in CAOV3 cells after piR-26441 overexpression, which was contrary to that after knockout. g R01 fluorescent probe results showed that overexpression of piR-26441 coupled with metformin significantly decreased the oxygen consumption rate in CAOV3 cells. h Flow cytometry analysis showed that overexpression of piR-26441 coupled with metformin significantly increased ROS levels in CAOV3 cells. i Comet assay showed DNA damage levels increased in CAOV3 cells after piR-26441 overexpression, which was contrary to that after knockout. All independent experiment repeated three times. Values are presented as the mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

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