Fig. 6: piR-26441 inhibits OC cell growth through the YTHDC1/TSFM signaling axis. | Cell Death & Disease

Fig. 6: piR-26441 inhibits OC cell growth through the YTHDC1/TSFM signaling axis.

From: piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1

Fig. 6

a Western blotting showed the protein levels of YTHDC1 decreased, and the protein levels of TSFM and NDUFB8 increased in piR-26441-overexpressing cells after YTHDC1 knockdown. b After treatment with actinomycin D (5 μg/ml) for 0, 1, 2, and 3 h, RT-qPCR assay showed the mRNA half-life of TSFM was prolonged in piR-26441-overexpressing cells after YTHDC1 knockdown. c qRT-PCR results showed the RNA levels of TSFM increased in piR-26441-overexpressing cells after YTHDC1 knockdown. d Western blotting showed TSFM and NDUFB8 protein levels increased in piR-26441-overexpressing cells after TSFM overexpression. e Flow cytometry analysis results showed ROS levels decreased in piR-26441-overexpressing cells after YTHDC1 knockdown and TSFM overexpression. f Comet assay showed DNA damage levels decreased in piR-26441-overexpressing cells after YTHDC1 knockdown and TSFM overexpression. YTHDC1 knockdown and TSFM overexpression g increased cell viability and h decreased apoptosis of piR-26441-overexpressing cells. All independent experiment repeated three times. Values are presented as the mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

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