Fig. 6: MLPH promotes primary cilia formation upon Gln deficiency.
From: Melanophilin-induced primary cilia promote pancreatic cancer metastasis
A IFT43 level positively correlated with MLPH expression. Scatter plot showed the relationship between genome-wide co-expression correlations for IFT43 and MLPH in pancreatic cancer tissues of NCKUH. (B-E) MLPH regulated primary ciliogenesis. B Depletion of MLPH decreased primary cilia formation in -QQ PANC-1 cells. Quantitative results of frequency of ciliated cells in -QQ PANC-1 cells infected with lentivirus containing shRNA against scramble control depletion -QQ (Scr) and MLPH (#1 and #2). C Overexpression of MLPH induced primary ciliogenesis. Quantitative results of frequency of ciliated cells in -QQ cells transfected with GFP or GFP-tagged MLPH. D MLPH localized to the base of primary cilia. Double staining of Prt and -QQ PANC-1 cells with antibodies against acetylated tubulin (Ac-tub, red) and MLPH (green). DNA was stained with DAPI (blue). Scale bar, 10 µm. E MLPH localized to centrosome. Double staining of Prt and -QQ PANC-1 cells with antibodies against gamma-tubulin (γ-tub, orange) and MLPH (green). DNA was stained with DAPI (blue). Scale bar, 10 µm. The localization of MLPH on centrosome from (F–G) interphase to (H–J) mitosis. Double staining of -QQ PANC-1 cells with antibodies against MLPH (green) and acetylated tubulin (Ac-tub, red). DNA was stained with DAPI (blue). Scale bars, 10 μm. Insets are magnification of centrioles. K Schematic representation of the expression of centriolar MLPH from interphase to mitosis. Data are represented as the mean ± SD of three independent experiments. ***P < 0.001.
