Fig. 1: AR is expressed in metastatic melanoma and its ligand activation prevents NK cell-mediated killing.
From: Role of the androgen receptor in melanoma aggressiveness
A AR gene expression levels in primary melanoma and in melanoma metastatic specimens (n = 481). AR was higher expressed in metastatic lesions (blu; n = 368; median 1.96) than in primary tumors (red; n = 103; median 1.33). One-way ANOVA with p = 0.03271 (f = 2.943). B WB analysis for AR in the indicated cell lines. Tubulin was revealed as a loading control. Representative experiment (left) and statistical analysis (right) of cytotoxicity assays done in untreated (white dots and bars) or 10 nM R1881-treated (black dots and bars) AR positive WM266-4 (C), AMM16 (D), Mel-CAL (E), LCM-Mel (F) and AR negative MEL-STA (G) cells with NK cells used at different E:T ratios. C–G Data from at least three independent experiments (n = 3) were represented as means ± SD. **p-value < 0.01; *p-value < 0.05.
