Fig. 4: AR and ADAM inhibition impairs the MICA shedding and the androgen-induced melanoma escape from NK cells.
From: Role of the androgen receptor in melanoma aggressiveness
A, B Cells were unstimulated or stimulated for 6 h with the indicated compounds. A Lysate proteins were analyzed for MICA/B expression using the anti-MICA/B antibody. The filter was re-probed using anti-GAPDH antibody, as loading control. B sMICA was quantified by ELISA. Data from three (n = 3) different experiments are expressed as mean ± SD; *p < 0.05. Representative experiment (C, E, G) and statistical analysis (D, F, H) of cytotoxicity assays performed in untreated or treated AMM16 (C, D), WM266-4 (E, F) and LCM-Mel (G, H) cells co-coltured for 6 h with NK cells used at different E:T ratios (50:1; 25:1). D, F, H Data derived from different experiments (n = 3) or (n = 4) are expressed as mean ± SD; *p < 0.05, **p < 0.01.
