Fig. 5: AR and ADAM inhibition impairs the androgen-induced melanoma escape from NK cells.
From: Role of the androgen receptor in melanoma aggressiveness
A, C AMM16-derived spheroids were untreated or treated as indicated in the figure and stained. Images of spheroids before (A) and after (C) the NK cells addition are presented. The green fluorescence from the acridin orange (AO) staining indicates total cells, while the red fluorescence from the propidium iodide (PI) staining indicates dead cells. C Spheroids were co-coltured for 4 h with NK cells. Images are representative of three different experiments. Bar, 100 µm. B, D The graphs represent the dead cells/total cells. Values of dead and total cells were analyzed using NIH Image J. They derive from red fluorescence mean/green fluorescence mean intensity and are expressed as mean ± SD of 3 different experiments (n = 3); *p < 0.05.
