Fig. 6: AR ligand-stimulation promotes migration and invasiveness of melanoma cells.
From: Role of the androgen receptor in melanoma aggressiveness
AMM16 (A, F), WM266-4 (B, G), MEL-CAL (C, H), LCM-Mel (D, I) and MEL-STA (E, L) cells were left unchallenged or challenged with R1881, in the absence or presence of Bicalutamide (Bic). Cells were allowed to migrate (A–E) or invade (F–L), counted and data are expressed as fold increase. AMM16 (M), WM266-4 (N) and MEL-CAL (O) cells were transfected with siRNA Alexa Fluor 488 along with control siRNA (siRNA ctrl) or siRNA AR (siRNA AR). Lysate proteins were analyzed by WB using the indicated antibodies (left panels in M–O). Cells were left in the absence or presence of R1881 and then allowed to migrate. Migrating cells were scored and data expressed as fold increase. In A–O, data are expressed as mean ± SD of 3 different experiments (n = 3); *p < 0.05; **p < 0.01.
