Fig. 1: UVB irradiation simultaneously upregulated indicators of pyroptosis, apoptosis and necroptosis in mice keratinocytes. | Cell Death & Disease

Fig. 1: UVB irradiation simultaneously upregulated indicators of pyroptosis, apoptosis and necroptosis in mice keratinocytes.

From: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

Fig. 1

A Flowchart of WT mice exposed or not exposed to low-dose (215 mJ/cm2) or high-dose (430 mJ/cm2) of UVB irradiation, and sacrificed at 48 or 72 h after UVB irradiation. B Photos of mice dorsal skin after different UVB irradiation doses and times (n = 4). C The scores of UVB damage of mice skin after different UVB irradiation doses and times (n = 4). D Representative images of H&E staining and TUNEL staining of mice skin sections after different UVB irradiation doses and times. The statistic of epidermal thickness and average optical density of TUNEL positive staining were analyzed (n = 4). E Representative images of MPO, MMP9, F4/80, CD4, and CD8 immunohistochemical staining in control mice and 430 mJ/cm2 UVB-irradiated mice at 72 h. The statistic of average optical densities of MPO, MMP9, F4/80, CD4, and CD8 positive staining were analyzed (n = 4). F Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL were detected by western blotting assay in epidermal lysates of control, 215 mJ/cm2 UVB 72 h, and 430 mJ/cm2 UVB 72 h groups of mice (sacrificed on the same day). The relative protein levels of the above proteins were compared with ACTB as quantification (n = 3). The scale bar in black is 100 μm for 10× magnification in D and E. The scale bar in yellow is 20 μm for 40× magnification in D. *P < 0.05. **P < 0.01. ***P < 0.001. ns not significant. GSDMD-FL GSDMD full length. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

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