Fig. 2: UVB irradiation simultaneously upregulated indicators of pyroptosis, apoptosis and necroptosis in human keratinocytes. | Cell Death & Disease

Fig. 2: UVB irradiation simultaneously upregulated indicators of pyroptosis, apoptosis and necroptosis in human keratinocytes.

From: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

Fig. 2

A Flowchart of acute UVB injury model in human skin explants. B Images of H&E staining of human skin explants from 3 samples. C Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL were detected by western blotting assay in epidermal lysates of human skin explants from 3 samples. The relative protein levels of the above proteins were compared with ACTB as quantification (n = 3). D Representative images of immunohistochemical staining of N-GSDMD, cleaved PARP, and p-MLKL in human skin samples from sun-exposed and non-exposed areas. The average optical densities of N-GSDMD, cleaved PARP, and p-MLKL positive staining were statistically analyzed (n = 12). The scale bar in black is 100 μm for 10× magnification in B and D. The scale bar in yellow is 20 μm for 40× magnification in B and D. *P < 0.05. **P < 0.01. ***P < 0.001. ns not significant. GSDMD-FL GSDMD full length. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

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