Fig. 4: Inhibiting caspase-3-mediated apoptosis alone did not decrease UVB-induced keratinocyte death. | Cell Death & Disease

Fig. 4: Inhibiting caspase-3-mediated apoptosis alone did not decrease UVB-induced keratinocyte death.

From: ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury

Fig. 4

A Flowchart of Ac-DEVD-CHO treatment or PBS treatment for acute UVB skin injury model in WT mice (48 h after 430 mJ/cm2 UVB). B Representative photos of mice receiving Ac-DEVD-CHO or PBS treatment 48 h after UVB exposure. C UVB skin damage of Ac-DEVD-CHO or PBS treated mice was evaluated by scores (n = 8). D Representative images of H&E staining and TUNEL staining of skin sections from Ac-DEVD-CHO or PBS treated mice after UVB irradiation. The statistic of epidermal thickness and average optical density of TUNEL positive staining were analyzed (n = 8). E Representative images of MPO, MMP9, and F4/80 immunohistochemical staining in Ac-DEVD-CHO or PBS treated mice after UVB irradiation. The statistic of average optical densities of MPO, MMP9, F4/80 positive staining were analyzed (n = 8). F Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, and p-MLKL in the epidermis of Ac-DEVD-CHO or PBS treated mice were detected by western blotting assay. The relative protein levels of the above proteins were compared with ACTB as quantification (n = 3). The scale bar in black is 100 μm for 10× magnification in D and E. The scale bar in yellow is 20 μm for 40× magnification in D. *P < 0.05. **P < 0.01. ns not significant. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.

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