Fig. 5: Blocking MLKL-mediated necroptosis alone did not decrease UVB-induced keratinocyte death.

A Photos of keratinocyte-specific Mlkl cKO mice (Krt14^Cre/+-Mlkl^flox/flox) and control mice (Krt14^+/+-Mlkl^flox/flox) at 48 h after exposure or non-exposure to 430 mJ/cm² UVB irradiation (n = 5). B UVB skin damage of Krt14^Cre/+-Mlkl^flox/flox mice and Krt14^+/+-Mlkl^flox/flox mice was evaluated by scores (n = 5). C Representative images of H&E, TUNEL, MPO, MMP9, and F4/80 staining in Krt14^Cre/+-Mlkl^flox/flox mice and Krt14^+/+-Mlkl^flox/flox mice after exposure or non-exposure to UVB irradiation. Statistical analysis of epidermal thickness and average optical densities of TUNEL, MPO, MMP9, F4/80 positive staining were shown (n = 5). D Proteins of N-GSDMD, cleaved PARP, cleaved caspase-3, MLKL and p-MLKL in the epidermis of Krt14^Cre/+-Mlkl^flox/flox mice and Krt14^+/+-Mlkl^flox/flox mice were detected by western blotting assay. The relative protein levels of the interested proteins were compared with ACTB as quantification (n = 3). The scale bar is 100 μm in C. *P < 0.05. **P < 0.01. ***P < 0.001. ns not significant. N-GSDMD N-terminal GSDMD. p-MLKL phosphorylated MLKL.