Fig. 2: MAPK4 promotes the differentiation of marginal zone (MZ) B cells. | Cell Death & Disease

Fig. 2: MAPK4 promotes the differentiation of marginal zone (MZ) B cells.

From: MAPK4 inhibits the early aberrant activation of B cells in rheumatoid arthritis by promoting the IRF4-SHIP1 signaling pathway

Fig. 2

A–C Flow cytometric analysis of the percentages and absolute numbers of bone marrow B cell subpopulations in WT and MAPK4 KO mice, including pre-pro-B cells (A), pro-B cells (B), early pre-B cells (C), late pre-B cells (D), immature B cells (E), and recirculating B (F) cells (n = 6). D, E Flow cytometric analysis of proportions and absolute numbers of splenic B cells in WT and MAPK4 KO mice (n = 6). F–N Flow cytometric analysis of peripheral B cell subpopulations in WT and MAPK4 KO mice (n = 6). The proportions and absolute numbers of FO (F, G), T1 (F, H), T2 (F, I), B1a (J, K), B1b (J, L) and MZ (M, N) cells were compared between WT and MAPK4 KO mice. O-P Proportion of MZ area in the lymphoid follicle area, analyzed by H&E staining of the spleen (n = 16). Scale bar = 200 μm. Q Immunofluorescence staining of spleen MZ cells from WT and MAPK4 KO mice. Scale bar = 25 μm. R, S Proportions and absolute numbers of splenic GC B cells under unimmunized conditions were detected and compared between WT and MAPK4 KO mice (n = 6). T Serum anti-dsDNA antibodies titers in WT and MAPK4 KO mice (n = 20). All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

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