Fig. 3: ACh-evoked unitary events in muscle fibers of WT and KO mice.

A Image of mouse hindlimb with flexor digitorum brevis (FDB) evidenced (white). B Representation of typical ACh-evoked unitary events recorded in visually identified NMJ on fibers from mice of the indicated strain. C Representative image of histogram of the amplitude of unitary events at 0 mV pipette potential. D Average values of slope conductance’s per strain (left), open time (centre) and closed duration (right) of the events of recorded patches. Measurements were made in 8–16 fibers as indicated (3 WT, 5 Sgcb KO mice). E Plot of the unitary amplitude vs. pipette potential in the same single fibers, with the best fitting lines. Arrowheads represent the reversal potentials (V₀). On the right, histogram of average measurements of reversal potentials. F Representative images of Indo immunofluorescence on WT and Sgcb KO female fibers; free Indo (red) and bound Indo (green), the fibers are outlined with a white dashed line. On the right, histogram relative to Indo-1 percentage. n = 9. G RNA levels of AchR subunits alpha (left), gamma (centre) and epsilon (right) on WT and Sgcb KO muscles. n = 6. H RNA levels of Agrin pathway; Lrp4 (left), Agrin (centre) and Musk (right) on WT and Sgcb KO muscles. n = 5. I Western blot representative images of Lrp4 and Musk. Gapdh was used as internal control. On the right, histogram with WB samples quantification. n = 5. All analyses were made using t-test, *p < 0.05; **p < 0.01; ***p < 0.001.