Fig. 2: Endothelial PCDH17 promotes metastasis in colorectal cancer.
From: PCDH17 induces colorectal cancer metastasis by destroying the vascular endothelial barrier
A Survival was analyzed and compared between patients with high and low ECs expression of PCDH17 in Cohort 2 (n = 82, log-rank test, two-sided). HR, hazard ratio. B, C PCDH17 mRNA expression level by UALCAN showing (B) nodal metastasis and (C) cancer status. Differences in significance were marked (Mann-Whitney U test). Data are presented as mean ± SD. D Representative immunofluorescence staining images of PCDH17 (red), CD31 (green), and DAPI (blue) in a CRC tissue microarray. The microarray were stained by CD31 antibody to identify blood vessel lumen. Scale bars, 50 μm. E Percentage of cells positive for both PCDH17 and CD31 among CD31+ cells in patients with CRC with (n = 32) or without distant metastases (n = 34). F Bioluminescence at 14 and 56 d post-injection of MC38-luciferase. G Quantification of the photon flux ratio per mouse at each time point (n = 4). H Imaging of metastatic nodules in liver and lung specimens from metastatic models established by spleen injection of MC38 cells. I,J Hematoxylin and eosin staining of mouse liver or lung with metastases (Left). Quantification of the metastatic nodules area per mouse (Right) (n = 4 mice per group). * P < 0.05, *** P < 0.001.
