Fig. 4: PCDH17 induced vascular endothelial barrier damage.
From: PCDH17 induces colorectal cancer metastasis by destroying the vascular endothelial barrier
A Bubble map of top 20 pathways for all DEGs by GO enrichment analysis. B The mRNA expression of PV1 were detected by RT-qPCR in HUVECs overexpressing or with or knocked down PCDH17. C Diffusion of Evans blue dye (EBD; 0.67 mg/ml) across membranes with pore diameters of 3 µm, with a monolayer of the HUVECs overexpressing PCDH17, at 3 h postdiffusion onset in the presence of BSA (40 mg/ml). The concentration of Evans blue dye that diffused across the membrane was quantified by a microplate reader, and the data are presented as the mean values (n = 3). D Transendothelial migration assay: mCherry-HCT116 cells were seeded on HUVECs confluent monolayers infected with lvPCDH17 or lvGFP. E Representative merges of the mCherry-positive transmigrated cells (Left). Number of mCherry-positive transmigrated cells (Right). Results are representative of three independent experiments each group in triplicate and values are expressed as the mean ± SD. **P < 0.01, ***P < 0.001.
